Abcam, ab249810, Anti-IPT antibody [EPR12517] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal IPT antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12517,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249810 is the carrier-free version of
ab174308
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The biological target 'IPT' also known as isopentenyl transferase is an enzyme involved in the catalysis of certain biochemical reactions. This enzyme has a molecular mass of approximately 32 kDa and is often expressed in various plant tissues including roots shoots and leaves. In the context of its role IPT facilitates the transfer of the isopentenyl group to adenine leading to the biosynthesis of cytokinins which are essential hormones for plant growth and development.
Biological function summary
IPT plays a central role in cytokinin biosynthesis by converting dimethylallyl diphosphate (DMAPP) and adenosine monophosphate (AMP) into isopentenyl adenine a precursor of cytokinin. Cytokinins together with auxins regulate cell division differentiation and apical dominance. IPT is often found as a part of a complex network of enzymatic reactions contributing to these processes. Its activity profoundly affects plant morphology and physiology by influencing the allocation of resources and determining plant architecture.
Pathways
IPT fits into the cytokinin biosynthetic pathway collaborating with other enzymes involved in plant hormone regulation. It plays an important role in modulating the balance between cytokinin and auxin levels which controls many developmental processes. The enzyme is closely associated with the IPT family of proteins and interfaces with the homodomain transcription factors that regulate plant growth responses to environmental stimuli. This places IPT in pivotal intersection points of hormonal and stress response pathways in plants.
Research highlights the importance of IPT's manipulation in plant biotechnology for crop improvement and stress resistance. Its association with increased cytokinin production has shown benefits such as delayed leaf senescence and enhanced resistance to drought and pathogens. There are indirect links between IPT and proteins involved in disease resistance pathways such as the pathogenesis-related proteins. Exploiting IPT's function in agricultural settings can lead to better yield stability and reduced agricultural losses due to environmental stressors.