Abcam, ab249918, Anti-SIRT6 antibody [EPR5079(N)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal SIRT6 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR5079(N),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249918 is the carrier-free version of
ab176345
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The SIRT6 protein also known as Sirtuin 6 plays an important role in cellular regulation. It is a member of the sirtuin family of proteins which are NAD+-dependent deacetylases. SIRT6 weighs approximately 39 kDa and can be found in the nucleus of many cell types. It acts mainly in regulating cellular homeostasis by removing acetyl groups from histone proteins which affects gene expression. Its expression levels vary across different tissues and it is known to be active in metabolically active organs such as the liver and brain.
Biological function summary
SIRT6 influences DNA repair metabolism and inflammation. It participates in maintaining genomic stability by promoting base excision repair a critical DNA repair process. Moreover SIRT6 contributes to glucose homeostasis by influencing gluconeogenesis and glycolysis. This protein is not known to be part of any larger protein complexes but it interacts individually with other proteins to exert its biological effects.
Pathways
SIRT6 plays a significant role in two key biological pathways: DNA damage response and metabolism regulation. In the DNA damage response pathway SIRT6 works with other proteins like PARP1 to facilitate DNA repair under stress conditions. In the regulation of metabolism SIRT6 interacts with transcription factors like HIF1α which influences the expression of genes involved in glycolytic metabolism and glucose homeostasis.
SIRT6 has associations with cancer and aging-related diseases. SIRT6 has a protective effect against oncogenesis by maintaining genomic stability and regulating metabolic pathways that cancer cells exploit. Additionally its role in aging is connected to its ability to prevent age-related genomic instability and metabolic decline. In cancer SIRT6 interacts with proteins such as c-Myc and p53 influencing cell growth and apoptosis pathways. This highlights its potential as a therapeutic target for both cancer and age-related conditions.