Abcam, ab249988, Anti-MAT2A+MAT1A antibody [EPR10496] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MAT1A antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR10496,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:ICC/IF, WB, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab249988 is the carrier-free version of
ab177484
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MAT2A (methionine adenosyltransferase 2A) and MAT1A (methionine adenosyltransferase 1A) are enzymes that catalyze the formation of S-adenosylmethionine (SAM) from methionine and ATP. MAT2A has a mass of approximately 43 kDa while MAT1A is around 45 kDa. MAT2A is expressed in all tissues except the liver where MAT1A is the more common form. The liver abundantly expresses MAT1A positioning it as a major contributor to hepatic SAM production while MAT2A forms are often seen in peripheral tissues and in proliferating cells.
Biological function summary
MAT2A and MAT1A play roles in one-carbon metabolism and are essential for the synthesis of SAM a universal methyl donor involved in methylation reactions. As part of a catalytic complex these enzymes contribute to the regulation of cellular methylation status and impact polyamine synthesis antioxidant defense and nucleotide synthesis. The activities of MAT2A and MAT1A influence cellular growth and differentiation reflecting their significance in metabolism and gene expression regulation.
Pathways
MAT2A and MAT1A participate in the methionine cycle which intersects with the folate cycle to regulate methylation processes and homocysteine levels. They form part of the trans-sulfuration and transmethylation pathways essential for converting methionine to cysteine and for methyl group transfers. Their activities align with pathways involving methionine synthase which similarly contributes to methyl group transfer and cycling of methionine and folates.
Dysfunction in MAT2A and MAT1A associates with liver diseases and certain cancers. In liver disorders imbalance in SAM synthesis due to MAT1A deficiencies can lead to hepatic steatosis and fibrosis. In cancer upregulation of MAT2A supports tumor growth by providing SAM for enhanced methylation and proliferation of cancer cells. Related proteins like glycine N-methyltransferase also connect to these disorders through their roles in SAM metabolism and regulation.