Abcam, ab250071, Anti-ALG2 antibody [EPR12006(2)(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ALG2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12006(2)(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250071 is the carrier-free version of
ab178697
Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please
contact us
for more information.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ALG2 protein also known as asparagine-linked glycosylation 2 plays a role in the early steps of N-glycan precursor biosynthesis. ALG2 has a molecular mass of approximately 51 kDa and shows expression in multiple tissues including the liver and brain. The protein acts mechanically to facilitate the addition of the first mannose residue to the lipid-linked oligosaccharide (LLO) precursor in the endoplasmic reticulum a fundamental step in protein glycosylation processes.
Biological function summary
Certain processes depend on the actions of ALG2. This protein becomes part of the larger oligosaccharyltransferase complex where it ensures proper glycosylation of nascent proteins—a modification important for protein folding and stability. ALG2 modulates the efficiency of protein maturation by its role in glycan formation affecting how proteins interact within cellular processes. Defects in ALG2 lead to improper protein folding and cellular distribution.
Pathways
ALG2 functions as an important component in the protein glycosylation pathway and more specifically in the N-glycan assembly pathway. This protein interacts closely with other glycosylation enzymes like ALG12 which further add branches to the glycans once ALG2 completes its function. The interactions within this pathway ensure proper biological functionalization of glycoproteins facilitating diverse roles from cell signaling to immune recognition.
ALG2 mutations have known associations with congenital disorders of glycosylation (CDG) a group of diseases caused by defects in glycosylation. Such mutations can cause a spectrum of developmental and metabolic issues. ALG2 dysfunction linked to proteins like ALG9 in co-regulatory roles reveals the impact of glycosylation on cellular homeostasis. These insights into ALG2 give promise for potential therapeutic targets in managing CDG symptoms and advancing understanding of glycosylation-related pathology.