Abcam, ab250165, Anti-AKAP14 antibody [EPR12955] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal AKAP14 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12955,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:IP, WB, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250165 is the carrier-free version of
ab180160
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
AKAP14 also known as A-kinase anchor protein 14 plays an essential role in anchoring protein kinase A (PKA) to specific intracellular compartments. It weighs approximately 100 kDa. Researchers found it primarily in the centrosome and mitochondria. The protein enables the localization and functions of PKA which modulates various cellular processes. Through its capacity to tether PKA AKAP14 facilitates targeted phosphorylation of substrates impacting cellular signaling dynamics and structural integrity.
Biological function summary
AKAP14 helps organize and maintain the structural framework of cells by anchoring PKA in cellular compartments. As part of a larger protein complex AKAP14 interacts with other structural proteins facilitating their assembly and function. This function is important for maintaining centrosome integrity and proper mitochondrial function. The anchoring partners of AKAP14 ensure precise PKA activity where required affecting cell cycle regulation and energy metabolism.
Pathways
AKAP14 functions in PKA-dependent phosphorylation pathways that influence intracellular signaling. In coordination with other proteins it plays roles in the cAMP signaling pathway which regulates cell growth differentiation and apoptosis. AKAP14's interaction with proteins like RII the regulatory subunit of PKA enables the tight modulation of signaling pathways essential for these cellular processes. Another significant pathway involving AKAP14 is the mitotic spindle formation pathway where it ensures centrosome stability and chromosome segregation.
AKAP14's dysfunction is linked to specific mitochondrial-related disorders and certain types of cancer. Disruption in its function can result in cellular signaling imbalance contributing to mitochondrial myopathy due to impaired energy metabolism. Furthermore AKAP14's involvement in centrosome regulation associates it with cancers such as breast cancer where centrosome abnormalities are common. Mutations that affect its interaction with other centrosomal proteins like ASPM could lead to anomalies in cell division promoting oncogenic processes.