Abcam, ab250315, Anti-MRPL20 antibody [EPR12592] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MRPL20 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12592,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250315 is the carrier-free version of
ab181058
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL20 or mitochondrial ribosomal protein L20 is a component of the mitochondrial ribosome involved in protein synthesis within the mitochondria. MRPL20 weighs around 27 kDa and is key in the formation and function of the mitochondrial large ribosomal subunit. It is expressed ubiquitously with high levels in tissues that demand significant energy such as the brain heart and skeletal muscle.
Biological function summary
MRPL20 plays a role in protein assembly within mitochondria forming part of the 39S ribosomal subunit. It interacts with RNA and other ribosomal proteins to ensure proper mitochondrial protein translation. MRPL20 works in close relationship with other mitochondrial ribosomal proteins such as MRPL3 and MRPL15 facilitating efficient mitochondrial biogenesis and energy production.
Pathways
The MRPL20 protein is significant in the mitochondrial translation pathway which is important for respiratory chain complex assembly. It aids in encoding proteins necessary for oxidative phosphorylation. MRPL20 associates with proteins like MRPS12 and MRPS18A which participate in mitochondrial protein synthesis integrating into the broader mitochondrial function and energy production network.
MRPL20 is linked to mitochondrial dysfunction and related diseases such as mitochondrial encephalomyopathy and cardiomyopathy. These conditions can result from mutations affecting MRPL20 disrupting mitochondrial protein synthesis and impairing energy production. MRPL20 interacts with proteins like MFN2 which are also involved in mitochondrial maintenance and have roles in disorders related to energy deficits in the human body.