Abcam, ab250397, Anti-HNRPH2/HNRNPH2 antibody [EPR12171] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HNRPH2/HNRNPH2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB, ELISA and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR12171,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human, Mouse, Rat,
Applications:WB, IHC-P, ELISA, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250397 is the carrier-free version of
ab181171
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4 Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HNRPH2 also known as hnRNP H2 is a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. It assists in RNA binding and processing playing a role in pre-mRNA splicing and mRNA metabolism. HNRPH2 has a molecular mass of approximately 49 kDa. Researchers have identified its expression in various tissues with a notable presence in the nucleus where RNA processing occurs.
Biological function summary
HNRPH2 participates in critical cellular mechanisms involving RNA. It plays a role in hnRNP complexes which help in the nuclear-cytoplasmic transport of mRNAs. This protein aids in alternative splicing which allows cells to produce multiple protein variants from a single gene. By binding to specific sequences on RNA HNRPH2 influences the maturation and stability of RNA molecules ensuring accurate gene expression.
Pathways
HNRPH2 functions in the regulation of gene expression and RNA splicing pathways. It associates with the spliceosome which is essential in removing introns from pre-mRNA. This activity links it to processes like the alternative splicing pathway integral for creating protein diversity in cells. Interactions with other hnRNP proteins such as hnRNP A1 highlight its involvement in maintaining cellular RNA homeostasis.
Alterations in HNRPH2 are linked to neurological disorders and cancers. Mutations in the HNRPH2 gene have been associated with Bainbridge-Ropers syndrome a neurodevelopmental disorder suggesting the gene's role in neural development. Disruptions in its function may also relate to cancer where the abnormal splicing of mRNAs can promote tumorigenesis. HNRPH2's interaction with proteins such as TDP-43 implies its possible involvement in neurodegenerative diseases.