Abcam, ab250440, Anti-alpha COP I/COPA antibody [EPR14273(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal alpha COP I/COPA antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14273(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250440 is the carrier-free version of
ab181224
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Alpha COP I also called COPA protein or alpha COPS is a component of the COPI coatomer complex involved in intracellular transport. This protein weighs approximately 134 kDa. Alpha COP is mainly expressed in the Golgi apparatus and other intracellular organelles. It functions as part of the COPI complex which is essential for retrograde Golgi-to-ER transport of proteins and lipids. This complex also plays an important role in maintaining Golgi structure and functionality.
Biological function summary
The alpha COP participates in forming the cytosolic coatomer complex which contains seven different proteins. This complex is involved in the transport of vesicles within eukaryotic cells. Alpha COP helps in the recruitment and assembly of other coatomer subunits necessary for vesicle budding and cargo selection. The protein interacts with other vesicle-forming machinery components ensuring efficient segregation and transport of cargo molecules. Additionally COPA notes contribute to cell homeostasis through effectively gathering sets of transport proteins.
Pathways
Alpha COP influences the regulation of the secretory pathway and lipid biosynthesis. The coatomer proteins including alpha COP interact with Arf family proteins which are critical regulators of vesicle formation by promoting membrane curvature and binding cargo. Another pathway involving alpha COP is the endocytosis pathway which is important for the recycling of plasma membrane components. This pathway also connects alpha COP with other proteins related to clathrin-mediated vesicle trafficking.
Defects in alpha COP have links to hereditary disease known as COPA syndrome which manifests with autoimmune features and interstitial lung disease. Mutations in the COPA gene impair protein transport function leading to improper immune responses. Alpha COP's interaction with proteins in the autoimmunity pathways can further relate it to rheumatoid arthritis. It affects the immune system's regulatory mechanisms and shares interaction partners with other immune system proteins such as cytokines and transcription factors involved in inflammatory processes.