Abcam, ab250517, Anti-MRPL16 antibody [EPR14351-63] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal MRPL16 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14351-63,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250517 is the carrier-free version of
ab181840
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRPL16 also known as mitochondrial ribosomal protein L16 is a part of the mitochondrial ribosome which helps synthesize proteins within the mitochondria. MRPL16 has a molecular weight of around 36 kDa. Researchers find this protein in most tissues but it is especially abundant in energy-demanding tissues like the heart and skeletal muscles. As part of the large subunit of the mitochondrial ribosome MRPL16 interacts with other ribosomal proteins to help maintain functions in protein biosynthesis.
Biological function summary
The mitochondrial ribosome plays an important role in facilitating mitochondrial protein synthesis by translating mitochondrial mRNAs into proteins. MRPL16 acts as a component of this ribosome which consists of numerous proteins and ribosomal RNA (rRNA). This mitochondrial ribosomal protein aids in stabilizing the structure and function of the ribosome ensuring accurate translation of genetic information within mitochondria. Through this function MRPL16 supports cellular respiration and energy production by maintaining the synthesis of essential proteins required for mitochondrial function.
Pathways
The mitochondrial protein synthesis pathway is essential for producing key proteins involved in oxidative phosphorylation the main ATP-generating mechanism in cells. MRPL16 through its association with the ribosomal complex plays a role in coordinating the production of proteins in the respiratory chain complexes. Proteins like cytochrome c oxidase and ATP synthase depend on the mitochondrial ribosome's proper function. MRPL16 therefore indirectly supports these pathways as anomalies in mitochondrial protein synthesis can disrupt ATP production.
MRPL16 has links to conditions involving mitochondrial dysfunction such as mitochondrial myopathy and Leigh syndrome. Defects in mitochondrial protein synthesis and energy metabolism often lead to these disorders. The protein's association with other ribosomal proteins like MRPL21 and MRPL11 becomes significant as disruptions in these proteins can lead to misfolding or incomplete synthesis of critical mitochondrial proteins. Understanding MRPL16's role helps us study how mitochondrial dysfunction contributes to these diseases and devise targeted therapies.