Abcam, ab250974, Anti-U2AF1L4 antibody [EPR14349] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal U2AF1L4 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14349,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, Flow Cyt (Intra), ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250974 is the carrier-free version of
ab188582
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
U2AF1L4 also known as U2 small nuclear RNA auxiliary factor 1-like 4 is a protein with a mass of about 30 kilodaltons. It plays a role in RNA splicing acting at the early stages of spliceosome assembly. U2AF1L4 binds to the polypyrimidine tract of pre-mRNA introns facilitating the recruitment of U2 snRNP to the branch point sequence. Expression of U2AF1L4 occurs mainly in tissues that are rich in nucleic acids and RNA such as testis and certain types of cancer cells.
Biological function summary
U2AF1L4 supports the regulation of gene expression through its role in the spliceosome machinery. It may form complexes with other splicing factors such as U2AF2 that are part of the early stages of spliceosomal assembly. This action supports the precise removal of introns and positions the exons accurately for translation. The ability to differentiate splice sites ensures diversity in mRNA transcription which is essential for proper function of genes in diverse tissues.
Pathways
U2AF1L4 fits into the pre-mRNA splicing pathway. It operates closely with other splicing-associated proteins like SF1 to help in the selection and cleavage of splice sites. Additionally U2AF1L4 interacts within the spliceosome pathway contributing to the splicing outcomes that regulate gene expression and are necessary for maintaining normal cell growth and function.
Irregularities in U2AF1L4 have been linked to certain cancers. Aberrant splicing by U2AF1L4 might play a role in tumor progression where mutations in its coding genes could lead to mis-splicing events. It engages with other proteins implicated in cancer such as the splicing regulator SF3B1 suggesting a complex interplay in tumorigenesis. Understanding these relationships could help develop targeted therapies for related disorders.