Abcam, ab250975, Anti-Syntaxin antibody [EPR15139(B)] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Syntaxin antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15139(B),
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IHC-P, WB, ICC/IF, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250975 is the carrier-free version of
ab188583
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Syntaxin also known as HPC-1 or STX1A functions as an important component in the vesicle docking and fusion process particularly in neuronal exocytosis. This protein weighs approximately 33 kDa and has a significant presence in the brain especially in the synapses. Syntaxin belongs to the SNARE protein family and plays a pivotal role in facilitating the fusion of synaptic vesicles with the presynaptic membrane allowing neurotransmitter release. Its expression is not limited to nervous tissue but occurs in various other tissues reflecting its versatile roles in cellular activities.
Biological function summary
Syntaxin interacts with other SNARE proteins such as SNAP-25 and VAMP to form the SNARE complex essential for synaptic vesicle fusion and neurotransmitter release. This complex mediates the membrane fusion process vital for effective neuronal communication. Syntaxin exhibits interaction with several regulatory proteins like Munc18 which stabilizes its closed conformation critical in maintaining a reservoir of readily releasable vesicles. Its involvement in the SNARE complex marks its importance in cellular exocytosis and endocytosis.
Pathways
Syntaxin participates prominently in the neurotransmission and secretion pathways. It interacts within the neurotransmission pathway by associating with SNAP-25 and VAMP facilitating rapid neurotransmitter release in synaptic transmission. Additionally in the secretion pathway Syntaxin involvement extends to insulin release interacting with proteins like Munc18 and enhancing vesicular transport processes. Its action in these pathways demonstrates its fundamental role in cellular signaling and material transport.
Syntaxin's dysfunction has links to neurological conditions such as Alzheimer's disease and botulism. In Alzheimer's disease abnormal Syntaxin interaction may impair synaptic function and neurotransmitter release leading to cognitive deficits. Botulism caused by botulinum toxin disrupts Syntaxin-mediated vesicle docking leading to muscular paralysis. Related proteins like SNAP-25 also play a role in these conditions emphasizing the complex network within the SNARE pathway that Syntaxin operates within highlighting its significance in maintaining cellular homeostasis and function.