Abcam, ab250980, Anti-LIME antibody [EPR14713] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal LIME antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR14713,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab250980 is the carrier-free version of
ab188871
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
LIME also known as Lck-interacting molecule is a transmembrane adaptor protein with a molecular weight of approximately 30 kDa. LIME is expressed mainly in immune cells such as T cells B cells and NK cells playing a significant role in immune system signaling. Its structural features include several tyrosine-based signaling motifs that interact with lymphocyte-specific protein tyrosine kinases. These interactions support LIME's function as a mediator in signaling cascades within immune cells.
Biological function summary
The LIME protein functions by helping to organize and transduce signals involved in lymphocyte activation proliferation and differentiation. It serves as a scaffold that aggregates proteins involved in signaling pathways forming part of larger signal transduction complexes. LIME directly associates with Src family kinases influencing cell signaling related to the immune response. This characteristic enables it to modulate various intracellular processes especially those concerning leukocytes and their responses to external stimuli.
Pathways
LIME participates in the T-cell receptor (TCR) signaling pathway and B-cell receptor (BCR) signaling pathway both important for adaptive immunity. In the TCR signaling pathway LIME interacts with ZAP-70 protein aiding T-cell activation and response to antigens. In the BCR signaling pathway LIME associates with kinases to modulate B-cell responses. Through these interactions LIME plays a role in optimizing the immune system's function and its ability to respond to pathogens or altered cells.
LIME has been linked to immune-related conditions like autoimmunity and lymphoma. Aberrant expression or mutations in LIME can disrupt normal signal transduction potentially leading to autoimmune diseases due to improper immune cell activation. Similarly altered LIME signaling has associations with lymphomas where it may impact cell proliferation and survival pathways. Changes in LIME function could affect its interacting partners such as Src family kinases contributing to the dysregulated growth and survival of immune cells seen in these disorders.