Abcam, ab251093, Anti-JNK1 + JNK3 antibody [EPR16797-194] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal JNK1 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat, Dog, Cow, Xenopus tropicalis, Recombinant fragment, Recombinant fragment - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16797-194,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Rat, Cow, Dog, Human, Xenopus tropicalis, Mouse,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251093 is the carrier-free version of
ab191603
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The c-Jun N-terminal kinases 1 and 3 (JNK1 and JNK3) are members of the mitogen-activated protein kinase (MAPK) family also known as Stress-Activated Protein Kinases (SAPKs). JNK1 has a molecular mass of approximately 46-54 kDa while JNK3 has a mass of about 54 kDa. JNK1 is expressed in most tissues with high levels in heart brain and leukocytes whereas JNK3 is mainly expressed in the brain but also found in the testes and heart. These kinases play a role in signaling cascades that respond to stress stimuli such as cytokines ultraviolet radiation and reactive oxygen species.
Biological function summary
JNK1 and JNK3 regulate transcription factors and influence gene expression to modulate cellular processes. They are part of a complex network that includes other proteins like MAP kinase kinases (MKKs) which activate JNKs through dual phosphorylation. JNK1 and JNK3 control processes such as cell proliferation apoptosis and differentiation. Their activation can trigger the phosphorylation of c-Jun a component of the Activator Protein 1 (AP-1) transcription factor complex influencing cell fate decisions.
Pathways
JNK1 and JNK3 are involved in important signaling pathways such as the MAPK signaling pathway and the apoptosis signaling pathway. These pathways interact with other proteins like MKK4 and MKK7 which act upstream to activate JNKs. The JNK pathway helps the cell respond to stress and regulate apoptosis interacting with proteins such as Bcl-2 which is involved in many cellular responses including cell survival and death.
JNK1 and JNK3 have been associated with conditions such as neurodegenerative diseases and cancer. Abnormal activation of these kinases is linked to Alzheimer’s disease which involves protein misfolding and synaptic dysfunction and to various cancers due to their role in controlling cell death and proliferation. In these contexts they interact with disease-related proteins like tau in Alzheimer's disease and p53 in cancer affecting disease progression and therapeutic responses.