Abcam, ab251388, Anti-HCLS1 antibody [EPR16540] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal HCLS1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR16540,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251388 is the carrier-free version of
ab202900
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The hematopoietic cell-specific Lyn substrate 1 commonly known as HCLS1 or HS1 is a cytoskeletal protein involved in cell signaling. It has a molecular mass of approximately 75 kDa and is expressed predominantly in hematopoietic cells such as lymphocytes and myeloid cells. HCLS1 functions as an adaptor protein playing an important role in the reorganization of the actin cytoskeleton which is critical for cell motility and morphology.
Biological function summary
The protein is important for signal transduction processes in immune cells particularly during the immune response. HCLS1 becomes phosphorylated upon cell activation allowing it to associate with other signaling proteins. It forms complexes with various partners like Lyn kinase and other SH2-containing proteins facilitating cytoskeletal rearrangement and cellular interactions necessary for effective immune function.
Pathways
HCLS1 is involved in key signaling pathways such as the B-cell receptor (BCR) and T-cell receptor (TCR) signaling pathways. In these pathways it works alongside proteins like SLP-76 and Vav to propagate signals that lead to cellular activation and differentiation. HCLS1's ability to bind and interact with proteins in these pathways highlights its role in orchestrating cellular immune responses.
HCLS1 has been implicated in certain autoimmune conditions and cancers including chronic lymphocytic leukemia (CLL) and systemic lupus erythematosus (SLE). In these contexts aberrant expression or activity of HCLS1 can disrupt normal immune signaling leading to pathological conditions. Additionally its interaction with proteins such as Bcl-2 in CLL suggests a role in apoptosis regulation further linking HCLS1 to disease progression and treatment response.