Abcam, ab251450, Anti-ENDOGL1/ENGL antibody [EPR15672] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal ENDOGL1/ENGL antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR15672,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IPSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251450 is the carrier-free version of
ab206300
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ENDOGL1 also known as Endonuclease G-Like 1 (ENGL) serves as an important enzyme in the cell that cleaves RNA. Its mass is approximately 35 kilodaltons. ENDOGL1 expresses mainly in the mitochondria where it acts closely with other mitochondrial components. This expression is also observed in various tissues throughout the body indicating a broad functional scope. As a ribonuclease it participates in the degradation of RNA which affects the processing and maturation of mitochondrial RNA.
Biological function summary
The role of ENDOGL1 centers around its participation in the maintenance of mitochondrial RNA stability. ENDOGL1 functions within a larger mitochondrial complex contributing to RNA turnover and regulation. By engaging directly with the RNA it plays a significant role in controlling and managing RNA fragments within the mitochondria essential for normal mitochondrial operations. This function ensures the smooth synthesis of the components required for mitochondrial respiration and energy production.
Pathways
ENDOGL1 integrates into mitochondrial RNA decay and processing pathways working alongside key proteins like POLRMT and TFAM involved in mitochondrial transcription and replication. ENDOGL1 influences the integrity and efficiency of these pathways by controlling RNA fragment degradation. Its activity in these pathways supports mitochondrial homeostasis and helps manage the mitochondrial gene expression thereby facilitating various energy production processes such as oxidative phosphorylation.
ENDOGL1 has connections to mitochondrial disorders and certain types of cancers. Disruption in ENDOGL1 function can lead to mitochondrial dysfunctions which contribute to the development of mitochondrial myopathies. Additionally abnormal RNA processing by ENDOGL1 may relate to cancerous transformations as it could affect RNA stability involved in cell growth and division. These pathways position ENDOGL1 as a potential target for therapeutic interventions aiming to address mitochondrial defects and oncology-related conditions.