Abcam, ab251459, Anti-CEACAM1+CEACAM6 antibody [EPR19878] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal CEAM1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR19878,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:WB, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251459 is the carrier-free version of
ab206431
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
CEACAM1 also known as CD66a and CEACAM6 commonly referred to as CD66c are members of the carcinoembryonic antigen-related cell adhesion molecule (CEACAM) family. CEACAM1 is a glycoprotein with a molecular mass of approximately 120 kDa while CEACAM6 has a molecular mass around 90 kDa. Both proteins are expressed on the surface of epithelial cells leukocytes and certain tumor cells. They function as cellular adhesion molecules mediating homophilic and heterophilic interactions which play a role in cellular communication and tissue architecture.
Biological function summary
CEACAM1 and CEACAM6 participate in immune response modulation and tumor progression. CEACAM1 can form a complex through its long cytoplasmic tail interacting with signaling proteins such as SHP-1 and SHP-2 influencing cell proliferation and apoptosis. CEACAM6 lacks a cytoplasmic domain interacting mainly through its extracellular region impacting cell adhesion and migration. Both proteins exhibit inhibitory functions on immune responses affecting the activity of immune cells such as neutrophils and T cells.
Pathways
CEACAM1 and CEACAM6 interact within key signaling cascades such as those involving the Src family kinases and PI3K/Akt signaling. In the context of immune signaling CEACAM1 associates with PD-1 signaling pathways influencing immune checkpoint control and potentially attenuating T cell activation. CEACAM6 interacts with integrins which are essential for cell adhesion and signaling pathways that regulate cell survival and migration.
CEACAM1 and CEACAM6 have associations with cancer and inflammatory bowel disease. Overexpression of CEACAM6 correlates with several types of cancer including colorectal and pancreatic cancer where it influences tumor cell survival and metastatic potential. CEACAM1 plays a role in the immune tolerance breakdown seen in inflammatory bowel diseases affecting mucosal immune responses. Both proteins are linked through their interaction with the immune system's regulatory proteins including PD-L1 in cancer highlighting their potential as therapeutic targets.