Abcam, ab251496, Anti-Prothrombin antibody [EPR20159] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Thrombin antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IP and reacts with Human samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20159,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Human,
Applications:Flow Cyt (Intra), IP, ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251496 is the carrier-free version of
ab208589
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Prothrombin also known as coagulation factor II is an important protein in blood clotting with an approximate mass of 72 kDa. This protein is synthesized in the liver and is expressed mainly in the plasma as an inactive precursor of thrombin. Prothrombin plays a pivotal role in the conversion into active thrombin through factor X activation in the presence of ionized calcium and phospholipid surfaces. Thrombin then further acts to convert fibrinogen into fibrin leading to clot formation. Prothrombin also can interact with various cellular receptors influencing other pathways beyond coagulation.
Biological function summary
Prothrombin regulates hemostasis and is part of the prothrombinase complex along with factor Xa factor Va calcium ions and phospholipids. This complex allows for the precise regulation of thrombin generation on the surface of activated platelets. By controlling the formation of thrombin prothrombin affects not only blood coagulation but also influences cell proliferation inflammation and tissue repair. The careful balance of prothrombin activation is important to prevent excessive bleeding or clot formation.
Pathways
Prothrombin is an essential component of the coagulation cascade especially the intrinsic and extrinsic coagulation pathways. In these pathways it links with proteins such as fibrinogen and factor X contributing to the amplification and perpetuation of thrombin generation. Additionally thrombin produced from prothrombin engages in a feedback loop to further enhance factor V VIII and XI activation. Through these actions prothrombin helps maintain normal coagulation but must be tightly regulated to prevent thrombotic complications.
Prothrombin is implicated in conditions like prothrombin thrombophilia and liver disease. Prothrombin thrombophilia is a genetic disorder that increases the risk for venous thromboembolism due to the presence of the G20210A mutation in the prothrombin gene. Moreover impaired prothrombin synthesis can occur as a result of liver disease leading to a higher bleeding risk. In thrombotic conditions proteins like protein C and antithrombin work to combat excessive prothrombin-generated thrombin underlining the necessity for balance and regulation in hemostatic processes.