Abcam, ab251513, Anti-p53R2+RRM2 antibody [EPR20651] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal RRM2 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20651,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WB, ICC/IF, IHC-P, Flow Cyt (Intra)See reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab251513 is the carrier-free version of
ab209995
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The p53R2+RRM2 complex also known as the ribonucleotide reductase small subunit p53R2 and ribonucleotide reductase M2 plays a mechanical role in DNA synthesis and repair. The mass of p53R2 is approximately 41 kDa while RRM2 is around 45 kDa. Together they form a vital part of the ribonucleotide reductase enzyme which is essential for converting ribonucleotides to deoxyribonucleotides the building blocks of DNA. This complex is expressed in many tissues but shows increased levels in response to DNA damage reflecting its role in responding to cellular stress.
Biological function summary
This complex is involved in maintaining genomic stability by supporting DNA repair processes. The p53R2 subunit is inducible by p53 linking its activity to cell cycle regulation and DNA damage response pathways. While p53R2 is induced by DNA damage RRM2 is generally expressed throughout the cell cycle ensuring a constant supply of deoxyribonucleotides for DNA replication and repair. Together these subunits ensure that cells can quickly respond to repair needs and maintain DNA integrity during stress events.
Pathways
The p53R2+RRM2 complex sits at the crossroads of the DNA repair and replication processes. The DNA damage response pathway heavily involves the p53 protein which upregulates p53R2 to ensure deoxyribonucleotide supply in repair mechanisms. Beyond the DNA damage response this complex also connects with the cell cycle pathway through the regulation of RRM2. Cyclin-dependent kinases and other cell cycle regulators are associated with controlling the levels and activity of RRM2 coordinating DNA synthesis with cell division.
Dysregulation of the p53R2+RRM2 complex links to cancer and neurodegenerative diseases. Mutations or altered expression of p53R2 may contribute to tumorigenesis by failing to repair damaged DNA efficiently. In certain cancers RRM2 overexpression occurs supporting increased cell proliferation. Additionally defects in DNA repair mechanisms involving the p53R2 subunit contribute to neurodegenerative disorders where accumulated DNA damage in neuronal cells leads to pathology. The p53 protein through its regulatory influence connects the function of this complex to both cancer progression and neurological disorders.