Abcam, ab251808, Anti-MDFIC antibody

Size: 100µL
Rabbit Polyclonal MDFIC antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MDFIC aa 50-150.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:IHC-P, ICC/IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human MDFIC aa 50-150. The exact immunogen used to generate this antibody is proprietary information.Q9P1T7

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MDFIC also known as MyoD family inhibitor domain containing is a protein that inhibits MyoD family of transcription factors. The mass of MDFIC is approximately 28 kDa. It plays a role in regulating gene transcription by binding to DNA and interfering with MyoD family members' ability to activate target genes. Researchers have detected MDFIC expression in various tissues with higher levels in the heart skeletal muscle and brain. MDFIC influences cellular differentiation processes in these tissues.
Biological function summary
MDFIC serves as a negative regulator in muscle cell differentiation by forming a complex with other transcription factors. This complex modulates the transcriptional activity of genes that manage muscle development and regeneration. MDFIC is involved in maintaining the balance between cell proliferation and differentiation ensuring that cells do not differentiate prematurely. This function allows tissues to grow and repair effectively.
Pathways
MDFIC participates in the regulation of myogenesis and signal transduction pathways. It interacts with the MyoD family especially MyoD and myogenin to modulate muscle-specific gene expression. Myogenesis the process of muscle cell formation requires a delicate regulation where MDFIC affects the balance by inhibiting transcription factors' binding affinity. Additionally MDFIC bears functional relevance in pathways involving nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kB) where it shifts cellular responses to external stimuli like stress or inflammation.
MDFIC involvement extends to certain muscular dystrophies where dysregulation can lead to impaired muscle repair and degeneration. MDFIC's connection with MyoD family proteins is significant in these disorders contributing to the pathology of muscle weakness and wasting. Furthermore MDFIC has relevance in cardiac pathologies such as cardiac hypertrophy where its regulation mechanism impacts heart muscle cells' ability to cope with stress and workload. The interplay between MDFIC and NF-kB signaling in cardiac tissues affects inflammatory responses which plays a part in disease progression.