Abcam, ab252364, Human Progranulin ELISA Kit

Size: 1 x 96Tests / 1 x 384Tests / 10 x 96Tests
Human Progranulin ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify human progranulin with a sensitivity of 72.68 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step. - Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader - Different formats for different needs: 10x96-well plates for bulk orders and 384-well for higher throughput - Validated on a number of sample types including cerebrospinal fluid (CSF) - Need to design your own assay: we also offer the conjugation-ready antibody pair
Key facts
Detection method:Colorimetric,
Sample types:Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma,
Reacts with:Human,
Assay type:Sandwich (quantitative),
Sensitivity:= 72.68 pg/mL,
Range:156.25 - 10000 pg/mL,
Assay time:1h 30m,
Assay Platform:Pre-coated microplate (12 x 8 well strips)

Product details:
Human Progranulin ELISA Kit ab252364 is a rapid single-wash 90-min ELISA kit to measure Human Progranulin in cerebral spinal fluid (CSF), saliva, urine, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.
How the assay works
Human Progranulin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit is designed for the quantification of human Progranulin. The standard protein in this kit is mature full-length human Progranulin. Native signal was detected in serum, plasma (heparin), plasma (EDTA), plasma (citrate), cell culture supernatant, milk, saliva, and CSF samples. Spiked protein experiments were used to validate urine and CSF sample types. Cell extract and tissue extract samples have not been tested with this kit.
25% male and female urine samples from healthy donors were measured in duplicate. All values were below the detectable range of the assay.
Human Progranulin ELISA Kit ab252364 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Progranulin ELISA Kit ab252364
Human Progranulin ELISA Kit ab252364 has been used to develop a prognostic test for uveal melanoma
References:
Gustav Stalhammar et al. 2023, PMID: 36973672
Save your precious samples
Our 384-well Human Progranulin ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Progranulin Antibody Pair - BSA and Azide free
ab256708
- Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture)
ab259663
- Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector)
ab259664
Related and recommended products
Human Progranulin ELISA Kit ab252364 is commonly used to study inflammation, wound repair, and neurodegeneration.
Other products to measure neuronal cell health include:
- Human Glutathione Peroxidase 4 ELISA kit
ab304936
- Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]
ab125066
- Anti-MAP2 antibody [EPR22641-16]
ab254264
To measure progranulin in mice:
- Mouse Progranulin ELISA kit
ab213473

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary
Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.
Pathways
Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.
Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.