Abcam, ab252849, Anti-Pescadillo antibody [8E9]

Size: 100µg / 1mg
Rat Recombinant Monoclonal Pescadillo antibody. Suitable for WB, IF and reacts with Mouse, Human samples. Cited in 3 publications.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:8E9,
Isotype:IgG1,
Carrier free:No,
Reacts with:Mouse, Human,
Applications:WB, IFSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Pescadillo also known as Pes1 is a nucleolar protein with an approximate molecular mass of 110 kDa. It is expressed in the nucleolus of human cells and plays a significant mechanical role in ribosome biogenesis. The protein functions in the processing of the 47S pre-rRNA transcript into 18S and 28S rRNAs which are essential components of the small and large ribosomal subunits respectively. Pescadillo is also involved in cell proliferation indicated by its higher expression levels in rapidly dividing cells such as cancer cells.
Biological function summary
The function of Pescadillo extends beyond ribosome production. It forms a part of a larger complex involved in the maturation of pre-ribosomal RNA interacting with proteins like BOP1 and WDR12. This interaction signifies its role in the PeBoW complex important for proper cell division and growth. Pescadillo influences the cell cycle and has implications in regulating p53-dependent pathways which are critical for maintaining genomic stability.
Pathways
Pescadillo plays a part in the ribosome biogenesis pathway and p53 signaling pathway. In ribosome biogenesis it works alongside other proteins such as NPM1 and RPL5 to ensure accurate ribosome assembly necessary for protein synthesis. Within the p53 signaling pathway Pescadillo modulates cell cycle checkpoints preventing DNA damage from leading to cancerous growths through its modulation of p53 an important tumor suppressor.
Pescadillo's aberrant function or expression links to cancer and developmental disorders. Its overexpression commonly associates with breast cancer where it may serve as a biomarker due to its involvement in increased cell proliferation. Additionally mutations in genes regulating Pescadillo activity can lead to disorders in embryonic development as protein partners like RPL5 are implicated in these processes. Understanding its regulatory mechanisms offers potential therapeutic targets in oncology and developmental biology.