Abcam, ab253838, Human Glutathione Peroxidase 3/GPx-3 Antibody Pair - BSA and Azide free

Size: 10 x 96Tests
Human Glutathione Peroxidase 3/GPx-3 Antibody Pair - BSA and Azide free is a kit containing recombinant capture and detector antibodies in a carrier-free formulation for the measurement of Human Glutathione Peroxidase 3/GPx-3.
Key facts
Reacts with:Human,
Assay type:ELISA set,
Range:31.25 - 8000 pg/mL,
Assay Platform:Reagents

Product details:
Human Glutathione Peroxidase 3/GPx-3 is a matched pair of unconjugated
recombinant rabbit monoclonal
capture and detection antibodies used to quantify Human Glutathione Peroxidase 3/GPx-3 in sandwich ELISAs and many other pair-based applications.
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The pair can be used in variety of assays and platforms including but not limited to:
Sandwich ELISA
FRET/TR-FRET/HTRF
Meso Scale Discovery
Bead-based assays
AlphaLISA
/AlphaScreen
DELFIA
immunoassays
and Single Molecule Counting (SMC™) immunoassays
Multiplex
Our antibody pairs are supplied in a carrier-free format that is conjugation-ready:
Buffer free of BSA, sodium azide, and glycerol for higher conjugation efficiency.
Concentration of ~1 mg/ml as measured by the protein A280 method.
We can label antibodies for you: get in touch today to discuss how we can help accelerate your assay development with custom conjugation services.
Pairs are screened in biological samples, including plasma and serum, to ensure specificity in complex samples.
Please note:
The recommended antibody orientation is based on internal optimization in sandwich ELISA. Antibody orientation is assay dependent and needs to be optimized for each assay type.
The range provided for this antibody pair is based on initial sandwich ELISA validation data using recombinant protein. Performance and range of the antibody pair will depend on the specific characteristics of your assay, including standard protein selection.
We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibodies in other assays.
Antibody properties:
Capture antibody:
recombinant rabbit monoclonal (unconjugated) – 100 µg
Detector antibody:
recombinant rabbit monoclonal (unconjugated) - 100 µg
Concentration
: ~1 mg/ml
Storage buffer:
100% PBS
Liquid
Isotype:
Recombinant monoclonal antibodies offer several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free production
Meso Scale Discovery and MSD are registered trademarks of Meso Scale Diagnostics, LLC.
AlphaLISA, AlphaScreen, and DELFIA are registered trademarks of PerkinElmer, Inc.
Simoa is a registered trademark of Quanterix, Inc.
SMC is a registered trademark of Merck KGaA, Darmstadt, Germany.

Properties and Storage Information:
Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-+4°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Glutathione Peroxidase 3 also known as GPx-3 or 3GPx is an antioxidant enzyme important for protecting cells from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides. It is a member of the glutathione peroxidase family and has a molecular mass of approximately 23 kDa. GPx-3 is expressed mainly in the kidney and subsequently released into the bloodstream playing an important role in its extracellular antioxidant defense mechanism.
Biological function summary
GPx-3 helps maintain cellular redox balance and is involved in detoxifying harmful oxidative agents. It does not form part of any larger complex but works independently to reduce peroxides with the help of glutathione an important reducing agent. GPx-3 has significant activities in plasma and extracellular fluids indicating its role in protecting the body from oxidative stress away from cellular environments.
Pathways
GPx-3 functions in the glutathione metabolic pathway and the defense system against oxidative stress. It works together with proteins such as glutathione reductase to recycle oxidized glutathione back to its reduced form allowing continuous protection against reactive oxygen species. This pathway is essential for maintaining balance between oxidative stress and antioxidant defense in the organism.
GPx-3 has been linked to cardiovascular diseases and certain types of cancer. Low levels of GPx-3 in plasma have been associated with increased risk of thrombosis as antioxidant defenses are compromised. In cancer studies altered GPx-3 expression is often observed thought to affect the progression of disease by changing the cellular redox environment. Other proteins that interact with GPx-3 during disease conditions include thioredoxin and catalase both influencing and being regulated by redox states in diseased tissues.