Abcam, ab254389, Anti-LRRC8A antibody [EPR23402-29]

Size: 20µL / 100µL / 1mL
Rabbit Recombinant Monoclonal LRRC8A antibody. Suitable for IP, WB and reacts with Mouse, Rat, Human samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR23402-29,
Isotype:IgG,
Carrier free:No,
Reacts with:Rat, Mouse, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The LRRC8A protein also known as SWELL1 plays a mechanical role as a component of the volume-regulated anion channel (VRAC). It has a molecular mass of approximately 104 kDa. The protein functions in transporting chloride ions across the plasma membrane helping to maintain cellular osmotic balance. LRRC8A is widely expressed in many tissues with high levels in the kidney heart and brain indicating its broad functional relevance across different organ systems.
Biological function summary
LRRC8A forms heteromeric complexes with related proteins LRRC8B LRRC8C LRRC8D and LRRC8E. These complexes constitute the VRAC channels which are important for regulating cell volume in response to osmotic changes a process called regulatory volume decrease. LRRC8A's involvement in this process is essential for cell survival under fluctuating environmental conditions as it prevents cellular swelling and lysis by allowing ions and osmolytes to exit the cell.
Pathways
Researchers have linked LRRC8A to the PI3K-AKT signaling pathway and the apoptotic pathway. In the context of the PI3K-AKT pathway LRRC8A contributes to cell survival and growth regulation interacting with proteins like AKT. Within the apoptotic pathway through VRAC activity LRRC8A plays a role in apoptosis regulation facilitating the release of apoptogenic factors that trigger programmed cell death when necessary.
Researchers have connected LRRC8A to conditions such as ischemia-reperfusion injury and cancer. In ischemia-reperfusion injury inadequate regulation by LRRC8A can lead to excessive cell swelling and damage upon reperfusion making it a potential therapeutic target. In cancer its role in cell growth pathways implicates LRRC8A in the uncontrolled proliferation seen in malignancies. Its association with VRAC partners like LRRC8D underpins its involvement in these disorders.