Abcam, ab254723, Anti-RIOK1/RIO1 antibody

Size: 100µL
Rabbit Polyclonal RIOK1/RIO1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RIOK1 aa 50-200.
Key facts
Host species:Rabbit,
Clonality:Polyclonal,
Isotype:IgG,
Carrier free:No,
Reacts with:Human,
Applications:ICC/IF, IHC-PSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:Recombinant Fragment Protein within Human RIOK1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.Q9BRS2

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.2Preservative: 0.02% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
RIOK1 also known as RIO1 is an atypical serine/threonine kinase with a molecular mass of approximately 67 kDa. It plays a role in processing small ribosomal subunit biogenesis. The protein is expressed in various tissues including liver and heart. Unlike typical kinases RIOK1 does not rely on the classical ATP-dependent mechanism for its kinase activity. It participates in the phosphorylation of some proteins which is necessary for ribosome maturation.
Biological function summary
The kinase RIOK1/RIO1 is part of the ribosome biogenesis pathway which is essential for protein synthesis. It forms part of a larger dynamic complex that assists in the maturation and transport of ribosomal components from the nucleus to the cytoplasm. RIOK1 plays a role in the final steps of 18S rRNA processing helping in the maturation of the 40S ribosomal subunit. This function makes it important in cellular growth and proliferation processes.
Pathways
The kinase RIOK1/RIO1 is involved in the ribosome biogenesis pathway and the associated cell cycle progression. It interacts with several proteins like NOB1 and ENP1 which are important for small subunit maturation. The efficient function of these pathways is necessary for normal cellular function and division. These partnerships ensure the necessary quality control of new ribosome parts as they are built highlighting its critical role in maintaining cellular homeostasis.
Alterations in RIOK1/RIO1 expression have been connected to cancers and neurological disorders. Aberrant expression levels contribute to abnormal cell growth in various cancer types implicating its role in tumorigenesis. Furthermore the dysregulation of RIOK1 can influence the activity of proteins such as p53 which is an important player in managing cellular stress responses and apoptosis. This association suggests potential as a biomarker for certain oncological conditions.