Abcam, ab255603, Anti-cleaved C-terminal GSDMD antibody [EPR20859-147]

Size: 100µL / 1mL
Rabbit Recombinant Monoclonal GSDMD antibody. Suitable for WB and reacts with Mouse samples. Cited in 18 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR20859-147,
Isotype:IgG,
Carrier free:No,
Reacts with:Mouse,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Preservative: 0.01% Sodium azideConstituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage duration-1-2 weeks, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved C-terminal GSDMD also known as cleaved gasdermin D is a protein involved in cellular processes influencing cell death. It is a fragment derived from gasdermin D (GSDMD) after cleavage which is facilitated by inflammatory caspases. This cleavage removes an inhibitory domain allowing the active N-terminal to initiate its functions. The molecular weight of GSDMD is approximately 53 kDa. Cleaved GSDMD is often detected through methods like Western blot particularly in immune cells such as macrophages and monocytes.
Biological function summary
Cleaved C-terminal GSDMD contributes significantly to the process of pyroptosis a form of programmed cell death that is inflammation-driven. This fragmented form is important for forming pores in the cell membrane leading to cellular lysis and the release of pro-inflammatory signals. Gasdermin D including its cleaved form is not known to be part of a large complex on its own but acts autonomously after activation by other proteins like caspase-1.
Pathways
Cleaved C-terminal GSDMD participates actively in the inflammatory response particularly in the inflammasome pathway. The protein is closely linked to caspases like caspase-1 that activate it leading to pyroptotic cell death. This pathway also intersects with other innate immune components such as the NLRP3 inflammasome to magnify inflammatory signals and recruit additional immune cells to sites of infection or damage.
Cleaved C-terminal GSDMD plays a role in conditions featuring excessive inflammation like septicemia and inflammatory bowel disease (IBD). In these diseases aberrant activation and overproduction of cleaved GSDMD can magnify tissue damage through uncontrolled cell death and inflammation. Also in septicemia GSDMD interacts with proteins like caspase-11 which can exacerbate inflammatory cytokine release and worsen the disease course.