Abcam, ab2557, Anti-Neutrophil antibody [NIMP-R14]

Size: 50µg
Anti-Neutrophil antibody [NIMP-R14] (ab2557) is a rat monoclonal antibody detecting Neutrophil in IHC-Fr . Suitable for Mouse . - Over 250 publications - Trusted since 2003
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:NIMP-R14,
Isotype:IgG2b,
Light chain type:unknown,
Carrier free:No,
Reacts with:Mouse,
Applications:IHC-FrSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Epitope:The monoclonal antibody NIMP-R14 is highly specific for murine Ly-6G and Ly-6C.

Product details:
The Ly-6G/-6C locus encodes a family of Ly-6 proteins including Ly-6G and Ly-6C. Ly-6 antigens have a molecular weight between 15,000 and 18,000. Ly-6G is together with Ly-6C a component of the myeloid differentiation antigen Gr-1. Ly-6G is a GPI-anchored protein and is a good marker of peripheral neutrophils. Although predominantly presents on neutrophils, it is also expressed on a subset of eosinophils, differentiating premonocytes and plasmacytoid dendritic cells. Ly-6C is a monocyte/macrophage and endothelial cell differentiation antigen regulated by interferon gamma, and may play a role in the development and maturation of lymphocytes. It is expressed on bone marrow cells, monocytes/macrophages, neutrophils, endothelial cells, and T cell subsets. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. However, the physiological role of Ly-6G alone remains still unclear. The monoclonal antibody NIMP-R14 has been successfully used to stain polymorphonuclear (PMN) cells and monocytes for fluorescent activated cell sorting and in frozen and paraffin sections. Treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses. Furthermore, it has been shown to be useful for depletion of neutrophils in mice. It depletes neutrophils as soon as 6 hours after injection and up to 6 days.
Product Specifications
Anti-Neutrophil antibody [NIMP-R14] (ab2557) is a rat monoclonal antibody and is validated for use in IHC-Fr in mouse samples.
Anti-Neutrophil antibody [NIMP-R14] (ab2557) specifically detects Neutrophil (UniProt ID: Q63317; Molecular weight: 9kDa) and is sold in 50 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-Neutrophil antibody [NIMP-R14] (ab2557) has high sensitivity and specificity.
Anti-Neutrophil antibody [NIMP-R14] (ab2557) has been cited over 250 times in peer reviewed journals and is trusted by the scientific community.
Anti-Neutrophil antibody [NIMP-R14] (ab2557) has 25 independent reviews from customers.
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Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein G, Storage buffer-Preservative: 0.02% Sodium azideConstituents: 0.1% BSA, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle