Abcam, ab255977, Anti-PAX9 antibody [7C2] - BSA and Azide free

Size: 100µg / 1mg
Rat Recombinant Monoclonal PAX9 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Mouse samples. Cited in 1 publication.
Key facts
Host species:Rat,
Clonality:Monoclonal,
Clone number:7C2,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Mouse,
Applications:ICC/IF, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.,
Specificity:Does not cross react with other PAX proteins

Product details:
ab255977 is the carrier-free version of
ab28538
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PAX9 also known as paired box gene 9 is a transcription factor with a molecular mass of approximately 37 kDa. It belongs to the PAX family of proteins characterized by the presence of a paired box domain. Mechanically PAX9 plays a role in DNA binding and transcriptional regulation of target genes. It is expressed in developing teeth craniofacial regions limbs and parts of the neural crest. This expression pattern highlights PAX9’s involvement in early developmental processes.
Biological function summary
PAX9 contributes significantly to developmental patterning and organogenesis. It functions as a part of a transcription factor complex that coordinates the gene networks necessary for these processes. PAX9 interacts with other PAX family members and developmental regulators mediating the formation of structures such as teeth and skeletal elements. PAX9’s role in these processes ensures proper structural differentiation and morphogenesis during development.
Pathways
PAX9 plays an important role in the tooth development pathway and the Wnt signaling pathway. During tooth development it interacts with proteins like MSX1 and BMP4 guiding tooth patterning and morphogenesis. In the Wnt pathway PAX9 influences cell signaling pivotal for cell fate determination and embryonic development where it cooperates with Wnt proteins to modulate pathway-specific gene expression.
Mutations or dysregulation of PAX9 have been associated with conditions such as tooth agenesis and oral clefts. Tooth agenesis which involves missing teeth arises from disruptions in PAX9’s functionality during tooth development often involving MSX1 as well. Oral clefts such as cleft lip and palate also involve PAX9 interactions during craniofacial development. These associations emphasize PAX9's critical role in maintaining normal developmental functions and its impact on congenital anomalies.