Abcam, ab256132, Anti-Connexin 32 / GJB1 antibody [2A] - BSA and Azide free

Size: 100µg / 1mg
Mouse Recombinant Monoclonal Connexin 32 / GJB1 antibody. Carrier free. Suitable for WB and reacts with Rat, Mouse, Human samples. Cited in 1 publication.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:2A,
Isotype:IgG1,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab256132 is the carrier-free version of
ab78052
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Connexin 32 also known as GJB1 is a gap junction protein with a molecular mass of approximately 32 kDa. This protein facilitates direct intercellular communication by forming gap junction channels that allow ions and small molecules to pass between adjacent cells. Connexin 32 is expressed in various tissues including liver pancreas and many types of epithelial cells. Its expression ensures cells can synchronize their activities important for maintaining tissue homeostasis.
Biological function summary
Connexin 32 plays a significant role in maintaining cellular communication networks. This protein forms hexameric complexes called connexons which dock with connexons of adjacent cells to create functional gap junction channels. These channels are essential in processes such as maintaining homeostasis cell differentiation and synchronized contraction of tissues like cardiac muscle. The structural formation allows direct transfer of metabolites and signaling molecules highlighting its importance in multicellular organism function.
Pathways
Connexin 32 actively contributes to cellular communication and metabolic coordination. It participates in the phosphoinositide 3-kinase (PI3-K) pathway and the ERK/MAPK signaling pathway which are important for cell growth proliferation and apoptosis. Connexin 32 interacts with proteins like Connexin 43 with both proteins playing roles in channel assembly and intracellular signaling processes that influence cellular responses to external stimuli.
Connexin 32's dysfunction aligns with specific neuropathies. Mutations in the GJB1 gene often lead to X-linked Charcot-Marie-Tooth disease a disorder affecting peripheral nerves. This disease involves degeneration of nerve fibers disrupting muscle function and sensation and connects with proteins that share functional pathways like PMP22. Additionally Connexin 32 has implications in liver diseases where its reduced expression correlates with disrupted hepatocyte communication affecting liver function and regeneration.