Abcam, ab256474, Anti-Cleaved Caspase-7 antibody [EPR22840-25] - BSA and Azide free

Size: 100µg / 1mg
Rabbit Recombinant Monoclonal Caspase-7 antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications.
Key facts
Host species:Rabbit,
Clonality:Monoclonal,
Clone number:EPR22840-25,
Isotype:IgG,
Carrier free:Yes,
Reacts with:Mouse, Rat, Human,
Applications:IP, WBSee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Immunogen:The exact immunogen used to generate this antibody is proprietary information.

Product details:
ab256474 is the carrier-free version of
ab256469
Patented technology
Our RabMAb
technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to
RabMAb® patents
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production
For more information, read more on
recombinant antibodies
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our
conjugation kits
for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar
Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar
is a trademark of Fluidigm Canada Inc.

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Protein A, Storage buffer-pH: 7.2 - 7.4Constituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions-+4°C, Storage information-Do Not Freeze

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Cleaved Caspase-7 also known as CASP-7 or cleaved Caspase is a protein involved in the execution phase of apoptosis. Its molecular weight is approximately 34 kDa before activation. It belongs to the family of cysteine-aspartic proteases and originates from a precursor with higher molecular weight. Cleavage activates it facilitating its expression in various tissues especially in locations where apoptosis is a regular process such as the liver heart and brain.
Biological function summary
Cleaved Caspase-7 plays a role by executing apoptosis through cleaving cellular substrates after aspartic acid residues. It operates independently or as part of the apoptosome complex which includes other caspases and protein factors. This complex contributes to the orderly dismantling of cellular structures during programmed cell death ensuring the removal of damaged or unnecessary cells in a controlled manner.
Pathways
Cleaved Caspase-7 participates actively in the apoptosis signaling pathway and to a lesser extent in the inflammatory response. It acts downstream in the cascade initiated by pro-apoptotic signals wherein it shares significant functional similarities with Caspase-3. Together with Caspase-8 which acts earlier in the pathway these proteins form a network that ensures the fidelity of programmed cell death. Caspase-8 initiates the cascade leading to the activation of Caspase-7.
Cleaved Caspase-7 associates notably with cancer and neurodegenerative diseases. Dysregulation of its activity often linked through aberrant apoptosis can lead to the unchecked growth of cancer cells or excessive neuronal death as seen in disorders like Alzheimer's disease. Caspase-8 is often implicated in these pathologies as well highlighting their intertwined roles in cell death and survival pathways. Consequently studying cleaved Caspase-7 helps improve understanding of and develop therapeutic strategies for these significant health issues.