Abcam, ab256979, Human LMNA (Lamin A) knockout HeLa cell lysate

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LMNA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon4 and 1 bp deletion in exon4 and 22 bp deletion in exon4.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,Western blot,
Mutation description:Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon4 and 1 bp deletion in exon4 and 22 bp deletion in exon4.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-LMNA, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Western blot, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Lamin A also known as LMNA is a type of lamin protein integral to the nuclear architecture. It has a molecular weight of approximately 70 kDa. This protein localizes mainly to the nuclear lamina a dense fibrillar network inside the inner nuclear membrane found across various cell types. Lamin A serves as a structural scaffold mediating nuclear stability and rigidity. It also plays a role in chromatin organization supporting overall nuclear functions.
Biological function summary
The lamin molecule interacts with several nuclear components forming part of the nuclear lamina complex. It anchors chromatin material and regulates DNA replication and repair. Lamin A protein also modulates gene expression through interactions with transcription factors. Beyond structural support it influences cell cycle progression and differentiation impacting cellular mechanotransduction and signaling processes.
Pathways
Lamin A performs critical functions within the cell cycle and apoptotic pathways. Its interactions with the retinoblastoma protein (pRB) and other cyclin-dependent kinases control cell cycle checkpoints and progression. Moreover lamin A connects with proteins involved in signaling pathways like MAPK which relate to stress responses and cellular growth. These interactions highlight its dynamic involvement in maintaining cell health and proliferation.
Mutations in the lamin A gene associate closely with Hutchinson-Gilford Progeria Syndrome and Emery-Dreifuss Muscular Dystrophy. In these conditions altered lamin A protein affects nuclear shape and chromatin layout disrupting transcriptional regulation. The mutated lamin A protein interacts differently with binding partners like emerin contributing to muscle adipose tissue and multi-system abnormalities. This highlights the critical role lamin A plays in maintaining normal cellular function across life stages.