Abcam, ab257407, Human DAPK3 (ZIP Kinase) knockout HEK-293T cell lysate

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DAPK3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Product details:
Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-DAPK3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
ZIP Kinase also known as DAPK3 is a serine/threonine protein kinase with a molecular mass of approximately 52 kDa. It is expressed in a variety of tissues including the heart liver and skeletal muscle indicating its broad physiological importance. ZIP Kinase shows activity in phosphorylating specific substrates that influence cell structure and function playing a role in cellular processes.
Biological function summary
ZIP Kinase regulates apoptosis and autophagy impacting cell survival and homeostasis. It does not act in isolation but is part of a complex with other kinases and regulatory proteins. ZIP Kinase interacts with cytoskeletal proteins to mediate cell contraction and motility indicating its involvement in both normal cellular dynamics and stress responses.
Pathways
ZIP Kinase is involved in the apoptosis and autophagy pathways. Within the apoptosis pathway ZIP Kinase partners with proteins like DAPK1 influencing stress and injury responses. In the autophagy pathway it helps in regulating cellular degradation processes. These pathways allow ZIP Kinase to integrate signals that manage cell fate decisions collaboratively modulating cellular life and death.
ZIP Kinase associates with hypertension and certain types of cancer. In hypertension ZIP Kinase dysregulation may impact vascular smooth muscle cell contractility due to its connection with myosin light chain phosphorylation. In cancer altered ZIP Kinase activity may affect apoptosis linking it to aberrant cell proliferation. Its interaction with proteins like DAPK1 in both contexts underlines its role in pathological states where cell death and survival are disrupted.