Abcam, ab258505, Human MAP3K10 (MLK2) knockout HEK-293T cell lysate

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MAP3K10 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 8 bp deletion in exon1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 8 bp deletion in exon1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAP3K10, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MLK2 protein also known as MAP3K10 (Mitogen-Activated Protein Kinase Kinase Kinase 10) plays a role as a serine/threonine kinase. It weighs around 95 kDa and affects multiple intracellular signaling pathways. It is expressed in numerous tissues including the brain placenta and kidney. MLK2 features a catalytic domain typical of MAP3 kinases and a CRIB domain that interacts with small GTPases.
Biological function summary
This kinase influences cellular dynamics by partaking in the MAP kinase signal transduction cascade and is a component of signaling complexes. MLK2 is important in the regulation of cell proliferation differentiation and apoptosis. It primarily transduces signals originating from cell surface receptors through multi-step phosphorylation events affecting downstream effectors. MLK2 interacts with MAP kinase kinases (MKKs) to activate c-Jun N-terminal kinases (JNKs) and p38 pathways.
Pathways
MLK2 acts within the MAPK signaling and JNK pathways. It regulates responses to environmental stresses and cytokines therefore modulating cell fate. MLK2 has functional associations with proteins like JNKs and MKKs which play leading roles in these pathways. Furthermore it works in conjunction with other MAP3Ks to regulate MAPK signaling cascades tightly.
MLK2 shows associations with neurodegenerative diseases and cancer. Dysregulation of MLK2 kinase activity can contribute to the pathophysiology of Alzheimer's disease by affecting neuronal survival pathways. In various cancers altered MLK2 expression or function may lead to abnormal cell growth. These conditions often involve connections with proteins such as JNKs which are implicated in cellular stress responses and apoptotic pathways.