Abcam, ab258517, Human MKNK2 (MNK2) knockout HEK-293T cell lysate

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MKNK2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon2 and 4 bp deletion in exon2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 20 bp deletion in exon2 and 4 bp deletion in exon2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MKNK2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The MNK2 kinase also known as MAP kinase-interacting serine/threonine-protein kinase 2 is a protein that plays a critical role in regulating mRNA translation. It weighs approximately 51 kDa and is part of the larger mitogen-activated protein kinase (MAPK) family. This protein is expressed widely across various tissues including the brain and liver. Its primary function is to phosphorylate specific substrates involved in the process of gene expression control.
Biological function summary
MNK2 modulates stress responses by influencing protein synthesis. It is an integral component of the mRNA translation machinery. MNK2 phosphorylates the eukaryotic translation initiation factor 4E (eIF4E) a process that is fundamental for the translation of specific mRNAs. By regulating eIF4E activity MNK2 helps to control the translation of mRNAs involved in stress response and cellular growth.
Pathways
MNK2 is significantly involved in the MAPK signaling pathway and plays a role in the response to cellular stress and growth signals. This pathway includes MAP kinases like ERK which activate MNK2. Through its interaction with eIF4E MNK2 facilitates the synthesis of proteins that are important for cell survival and proliferation in response to extracellular signals. Its action links upstream signaling events influenced by MAP kinases to downstream processes affecting cell growth and stress response.
MNK2 has been implicated in cancer due to its role in the regulation of protein synthesis that supports cell proliferation. Abnormal MNK2 activity is linked to tumorigenesis particularly in cancers where eIF4E plays a role like breast and prostate cancer. Moreover its interaction with proteins such as eIF4E and MAP kinases highlights potential targets for therapeutic intervention. By modulating MNK2 activity researchers aim to control aberrant protein synthesis pathways driving these diseases.