Abcam, ab258521, Human MORF4L2 (MRGX) knockout HCT116 cell lysate

Size: 1Kit
MORF4L2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon5.
Key facts
Cell type:HCT116,
Species or organism:Human,
Tissue:Colon,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon5.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MORF4L2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
MRGX also known as Mas-related G protein-coupled receptor member X functions as a G protein-coupled receptor (GPCR) involved in sensing environmental stimuli and transducing signals inside cells. Researchers find this receptor in sensory neurons within the dorsal root ganglia and trigeminal ganglia. MRGX weighs approximately 37 kDa and plays a role in pain perception and modulation by interacting with various ligands. Understanding its mechanical function helps in exploring its wide applications in sensory biology.
Biological function summary
This receptor contributes to the modulation of nociceptive signals and influences pain sensation and response. MRGX does not form part of a complex; it functions independently to modulate pain pathways by interacting with peptides and other small molecules. It also regulates calcium ion concentrations within neurons which is essential for neurotransmission and signal propagation. The interaction of MRGX with these elements highlights its significant contribution to the sensory regulatory processes.
Pathways
Research associates MRGX with pain perception and sensory transmission pathways. It intersects with the neuropeptide signaling pathway influencing proteins such as substance P and calcitonin gene-related peptide (CGRP) both key players in pain signal pathways. MRGX modulates the activity of these signaling molecules affecting how the body interprets and reacts to painful stimuli. This receptor's involvement in these pathways demonstrates its impact on the physiological responses to pain.
MRGX is linked to conditions such as neuropathic pain and inflammatory pain. Alterations in MRGX function or expression can influence the severity of pain-related phenomena implicating it in these conditions. The association of MRGX with proteins like TRPV1 and Nav1.8 which are involved in pain pathways further solidifies its connection to these disorders. Understanding these interactions offers insights into potential therapeutic avenues for treating chronic pain syndromes.