Abcam, ab258545, Human NRBP2 knockout HeLa cell lysate

Size: 1Kit
NRBP2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NRBP2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
NRBP2 also known as Nuclear Receptor Binding Protein 2 functions mechanically as an adaptor protein implicated in signal transduction processes. It has a molecular mass of approximately 65 kDa. The expression of NRBP2 occurs mainly in the brain and testis suggesting a role in specialized cellular activities of these tissues. Its adaptor function facilitates interactions in cellular signaling cascades modulating responses to various stimuli.
Biological function summary
NRBP2 influences several cellular processes due to its role as part of a multiprotein complex. It participates in regulating cell proliferation and differentiation essential tasks for maintaining normal cellular homeostasis. NRBP2's involvement in these processes aids in controlling the balance between cell growth and death important for healthy cellular turnover.
Pathways
NRBP2 impacts the Wnt/β-catenin signaling pathway and JNK signaling pathway. These pathways play roles in developmental processes and cell stress responses. Within these signaling cascades NRBP2 interacts with proteins like GSK3β and c-Jun N-terminal kinases which modulate transcriptional regulation and cellular response to environmental cues integrating signals from various pathways.
NRBP2 associates with conditions such as schizophrenia and cancer. Its interaction with CDK4 and β-catenin proteins in cancer highlights its role in tumorigenic processes potentially contributing to abnormal cell division and growth. NRBP2's dysregulation in schizophrenia suggests a connection with neurological pathways that involve neurotransmitter signaling and synaptic plasticity.