Abcam, ab258604, Human PRKCI (PKC iota) knockout HeLa cell lysate

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PRKCI KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 5.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: Insertion of the selection cassette in exon 5.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PRKCI, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Protein Kinase C (PKC) iota also known by its gene symbol PRKCI is an atypical member of the PKC family. It has a molecular mass of approximately 74 kDa. PKC iota does not respond to calcium and is insensitive to diacylglycerol and phorbol esters which differentiates it from other classical PKC family members. The protein initiates cell survival and proliferation pathways and is primarily expressed in brain heart liver and skeletal muscle tissues indicating its extensive role across various biological functions.
Biological function summary
PKC iota acts as an important player in regulating cell polarity and protecting cells from stress-induced apoptosis. It forms part of the Par6-Par3-PKC iota complex which is important for maintaining cell polarity and directional migration. This complex is essential for neural tube closure during embryonic development and in establishing cell polarity across diverse cell types. PKC iota also contributes to the regulation of microtubule organization reinforcing its vital role in maintaining cellular architecture.
Pathways
PKC iota is important in the NF-kB signaling pathway and the PI3K/AKT pathway. In the NF-kB pathway PKC iota activates IkB kinase leading to NF-kB transcriptional activity which promotes cell survival. In the PI3K/AKT pathway PKC iota contributes to cell growth and survival working closely with AKT proteins. These pathways highlight its involvement in processes like proliferation survival and cellular response to external stimuli.
PKC iota has a significant connection to cancer and neurological disorders. Its overexpression has been observed in several cancers such as lung and prostate cancer where it is often associated with poor prognosis. PKC iota’s role in the maintenance of cell polarity and survival links it to malignant cell behavior. Additionally in neurological disorders like Alzheimer’s disease alterations in PKC iota activity have been related to synaptic dysfunctions. Connections to proteins such as AKT and NF-kB in these conditions highlight its potential as a therapeutic target.