Abcam, ab258610, Human PSME3 knockout HEK-293T cell lysate

Size: 1Kit
PSME3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PSME3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The PSME3 protein also known as PA28γ or REGγ is mechanically an essential proteasome activator that facilitates the degradation of ubiquitin-independent substrates. It has a molecular weight of approximately 29 kDa. PSME3 is mainly found in the nucleus and shows strong expression in various tissues including liver kidney and heart. The protein operates by binding to the 20S proteasome core and enhances the specificity of proteolytic activity.
Biological function summary
PSME3 regulates cell cycle progression and apoptosis. It is part of a larger multiprotein complex with the 20S proteasome core forming an important component in protein homeostasis. PSME3’s interaction with the proteasome core significantly reduces the accumulation of certain regulatory proteins by promoting their degradation. This function affects several cellular processes such as cell proliferation and DNA repair aiding the maintenance of cellular function and integrity.
Pathways
PSME3 plays a critical role in mediating the ubiquitin-proteasome pathway and the MDM2-p53 pathway. In the ubiquitin-proteasome pathway it assists in the controlled turnover of intracellular proteins which is pivotal for maintaining cellular protein quality. In the MDM2-p53 pathway it affects the stability of the p53 protein by enhancing the degradation of both MDM2 and p53 therefore modulating apoptosis and growth arrest in cells under stress or DNA damage conditions.
Disruptions in PSME3 function correlate with cancer and neurodegenerative disorders. Overexpression of PSME3 is often linked to oncogenesis due to its role in controlling the degradation of tumor suppressor proteins like p53. This protein is also implicated in Parkinson’s disease where its interaction with pathogenic proteins suggests it might contribute to the accumulation of misfolded proteins. PSME3 along with its pathways and related proteins offers potential as a target for therapeutic intervention in these diseases.