Abcam, ab258645, Human RRAGC knockout HeLa cell lysate

Size: 1Kit
RRAGC KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1 and Insertion of the selection cassette in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-RRAGC, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The RRAGC protein also known as Ras-related GTP-binding protein C weighs approximately 44.5 kDa. It functions mechanically as a GTPase cycling between active GTP-bound and inactive GDP-bound states. This protein is expressed in various tissues with noticeable presence in the brain and liver. RRAGC plays a role in cellular signaling and growth mechanisms by interacting with other members of the Ras superfamily.
Biological function summary
RRAGC forms a part of the heterodimeric Rag GTPase complex often teaming with its partner RRAGB. This complex acts as an important regulator in the mTORC1 signaling pathway influencing nutrient sensing and energy balance within cells. RRAGC's ability to toggle between different energy states allows it to contribute to cellular metabolism and response to external stress.
Pathways
RRAGC engages directly in the mTOR signaling pathway an important driver of cell growth and proliferation. It interacts closely with related proteins such as Raptor and mTOR kinase forming a bridge between amino acid availability and cellular growth signals. Additionally RRAGC's role connects to the autophagy pathway affecting cellular degradation processes and nutrient recycling under nutrient-deprived conditions.
The dysregulation of RRAGC's activity associates with conditions like cancer and neurodegenerative diseases. Alterations in its function may link to aberrant mTOR signaling contributing to uncontrolled cellular proliferation seen in cancers. Furthermore RRAGC and its interactions with proteins such as TSC2 (Tuberous Sclerosis Complex 2) highlight potential influences in neurodegenerative disease pathways shedding light on its involvement in conditions linked to cellular growth dysregulation.