Abcam, ab258655, Human SEC11A (SPC18) knockout HEK-293T cell lysate

Size: 1Kit
SEC11A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 31 bp deletion in exon 2.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 31 bp deletion in exon 2.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SEC11A, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SPC18 also known as Spindle Pole Body Component 18 is an integral protein component of the spindle pole body in yeast cells. This protein exhibits a molecular mass of about 18 kDa and is primarily expressed in yeast during cell division. SPC18 is essential as it facilitates and stabilizes the connection between microtubules and the spindle pole body which ensures proper chromosomal segregation during cell division.
Biological function summary
SPC18 functions as part of the yeast's spindle assembly complex. This complex plays an important role in regulating the organization and dynamics of spindle microtubules. SPC18 helps maintain spindle integrity and aids in anchoring microtubules to the spindle pole body. Its function is important for successful mitotic progression and ensures that chromosomes are accurately distributed into daughter cells.
Pathways
SPC18 is involved in the mitotic spindle assembly pathway. It interacts closely with proteins like Spc110 and Tub4 which are also part of the spindle pole body assembly. The mitotic pathway in which SPC18 plays a role ensures proper chromosome alignment and segregation during mitosis which is vital for genomic stability.
Impaired function of proteins homologous to SPC18 in humans could potentially relate to disorders such as cancer due to improper cell division and chromosomal instability. Dysregulation of proteins involved in the spindle pole body like human equivalents of Spc110 can lead to aneuploidy a condition often associated with malignant transformations. Therefore SPC18 through its homologs provides insights into mechanisms that might contribute to such diseases.