Abcam, ab25866, Anti-HIV1 Nef antibody [3F2]

Size: 100µg
Mouse Monoclonal HIV1 Nef antibody. Suitable for RipA, WB, ELISA and reacts with Recombinant full length protein - Human immunodeficiency virus samples. Cited in 6 publications.
Key facts
Host species:Mouse,
Clonality:Monoclonal,
Clone number:3F2,
Isotype:IgG1,
Carrier free:No,
Reacts with:Human immunodeficiency virus,
Applications:WB, RipA, ELISASee reactivity dataSee the reactivity data table below for information on validated species and application combinations.,
Epitope:Mapped to amino acids 31-35 (GAASR, HIV-1 HxB2).

Properties and Storage Information:
Form-Liquid, Purification technique-Affinity purification Immunogen, Storage buffer-pH: 7.4Preservative: 0.1% Sodium azideConstituents: PBS, Shipped at conditions-Blue Ice, Appropriate short-term storage conditions-+4°C, Appropriate long-term storage conditions--20°C, Aliquoting information-Upon delivery aliquot, Storage information-Avoid freeze / thaw cycle

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
HIV1 Nef also known as Nef HIV Nef protein and Nef HIV protein is a small myristoylated protein with a mass of approximately 27 kDa. It expresses predominantly in the cytoplasm of HIV-1 infected cells. HIV1 Nef plays an important role in viral pathogenicity by manipulating host cell machinery to favor viral replication and immune escape. The protein affects the host cell's plasma membrane and endosomal compartments where it modifies the trafficking of various cell surface molecules.
Biological function summary
Nef affects several cellular processes to promote viral persistence and disease progression. It disrupts normal cellular signaling and acts as part of larger protein complexes that interact with host proteins. For instance it is known for its interaction with proteins like AP-1 which Nef uses to alter the re-routing of MHC-I molecules to lysosomes. This results in reduced exposure of infected cells to the immune system and highlights its role in immune evasion.
Pathways
Nef engages in interference with immune and cellular signaling pathways. It tightly interacts with the PI3K/Akt signaling pathway important for cell survival and proliferation thereby assisting the virus in sustaining a hospitable cellular environment. Nef also partners with Src family kinases further supporting its functions in reprogramming cellular processes and enhancing viral replication.
HIV1 Nef is intimately related to HIV/AIDS pathogenesis. It contributes significantly to the immunodeficiency observed in infected individuals by modulating CD4 and CTLA-4 downmodulation affecting immune signaling and response. Furthermore Nef aligns with Tat protein in HIV-infected cells enhancing the pathological effects seen in the neurodysfunction often associated with disease progression illustrating its role in complex interactions that worsen HIV-related complications.