Abcam, ab258673, Human SLC25A22 (GC-1) knockout HEK-293T cell lysate

Size: 1Kit
SLC25A22 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 3 and 8 bp deletion in exon 3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-SLC25A22, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
GC-1 also known as guanylate cyclase 1 is an enzyme with an approximate molecular mass of 82 kDa. It functions mechanically by converting GTP to cGMP a critical second messenger in various cellular processes. GC-1 is expressed in a range of tissues including the brain and retina where it plays important roles in visual and neural functions. Its distribution indicates its involvement in both sensory perception and broader neurological activities.
Biological function summary
GC-1 plays an important role in the signal transduction pathways involving nitric oxide as part of a complex with protein partners. As a subunit of the soluble guanylate cyclase (sGC) complex it helps mediate the effects of nitric oxide by regulating cGMP levels. This activity is essential for promoting vasodilation and blood flow which is observed in the cardiovascular system. The GC-1 complex translates extracellular signals to intracellular responses affecting various physiological processes.
Pathways
GC-1 is a critical component in the nitric oxide signaling pathway where it facilitates communication between nitric oxide and cGMP-dependent pathways. It interacts closely with proteins such as endothelial nitric oxide synthase (eNOS) and is directly involved in the pathway regulating vascular tone and blood pressure. Additionally GC-1 is part of the phototransduction pathway in retinal cells influencing the conversion of light into electrical signals through its production of cGMP.
GC-1 has significant implications in cardiovascular diseases and certain eye disorders. For cardiovascular diseases GC-1 contributes to conditions related to hypertension and heart failure as it affects the nitric oxide-cGMP pathway's ability to mediate vascular relaxation. In the realm of eye disorders particularly retinitis pigmentosa impaired GC-1 function affects photoreceptor cells and visual signal transduction. GC-1's activity is linked to proteins like phosphodiesterase 6 (PDE6) which further highlights its role in maintaining visual acuity and retinal health.