Abcam, ab258717, Human THEMIS2 (ICB1) knockout A549 cell lysate

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THEMIS2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
Key facts
Cell type:A549,
Species or organism:Human,
Tissue:Lung,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.,
Disease:Carcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-THEMIS2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein ICB1 also known as Intermediate Complex B1 exhibits mechanical functions critical to cellular processes. With a molecular mass of approximately 50 kDa ICB1 is widely expressed in various tissues including the liver brain and muscles. This protein contributes to important mechanistic roles in cells influencing their structural and functional stability. Its distribution across different tissues indicates its involvement in diverse cellular functions.
Biological function summary
The role of ICB1 is linked to cellular signaling pathways and structural maintenance. It often forms part of a larger protein complex within the cytoplasm contributing to cytoskeletal integrity. Through its interactions ICB1 helps in the regulation of intracellular transport and communication. Its integration into multiprotein complexes highlights its importance in maintaining cellular architecture and communication networks.
Pathways
ICB1 participates in signaling cascades such as the MAPK and PI3K-Akt pathways. These pathways are essential for cell survival growth and differentiation. ICB1 interacts with proteins like Akt and ERK1/2 helping to mediate signals that influence cell fate decisions. Its role in these pathways highlights its importance in regulating cell proliferation and responses to external stimuli.
ICB1 has connections to neurodegenerative diseases and certain cancers. In Alzheimer's disease ICB1 is thought to influence the pathology through its interaction with tau proteins contributing to neurofibrillary tangle formation. In some types of cancer alterations in ICB1 expression affect cell cycle regulation promoting unrestrained growth. Understanding the involvement of ICB1 with proteins like tau and cyclin-dependent kinases in these contexts is important for therapeutic development.