Abcam, ab258763, Human WDR6 knockout HeLa cell lysate

Size: 1Kit
WDR6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 19 bp deletion in exon 1 and 1 bp insertion in exon 1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-WDR6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
WDR6 also known as WD Repeat Domain 6 is a protein with a mass of approximately 115 kDa. This protein contains multiple WD40 repeats which facilitate protein-protein interactions. WDR6 is widely expressed across various human tissues including brain skeletal muscle heart and testis. Its presence in multiple tissues suggests a critical role in cellular functions.
Biological function summary
WDR6 is involved in regulatory systems that control cell growth and division playing a role in translational regulation. WDR6 does not function alone; it interacts with specific complexes to modulate the ribosomal protein synthesis process. It interacts with nutrient-sensing pathways and may work with proteins like RPS6 leading to changes in protein translation and cell growth regulatory mechanisms.
Pathways
Scientists see WDR6 as part of the mTOR signaling pathway which is important for controlling cell growth and proliferation. WDR6's function associates it with ribosomes and components involved in protein synthesis regulation such as S6K1 and 4EBP1. These interactions enable WDR6 to assist in integrating signals from nutrient and energy status to regulate cell growth.
Researchers have observed a connection between WDR6 and cancer owing to its role in cell growth control. Dysregulation of the mTOR pathway with which WDR6 is involved often leads to abnormal cell proliferation seen in some cancers. Additionally variations in WDR6 expression and function may relate to neurodegenerative diseases where proteins like mTORC1 and p70S6K could be involved in pathways affecting neuronal survival and function.