Abcam, ab258774, Human ABCF3 knockout HeLa cell lysate

Size: 1Kit
ABCF3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-ABCF3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The ABCF3 protein also known as ATP-binding cassette sub-family F member 3 functions in cellular processes involving ATP binding and hydrolysis. This protein weighs approximately 95 kDa. It shows expression in several tissues including the liver kidney and testis. As part of the ATP-binding cassette (ABC) family it lacks a transmembrane domain but participates significantly in nucleotide binding.
Biological function summary
ABCF3 plays a role in cellular stress response and protein synthesis regulation. It often forms part of a multi-protein complex aiding the translation process by interacting with ribosomal subunits. This interaction suggests an involvement in the regulation of translation initiation which is important for effective protein synthesis. Additionally ABCF3 may have a role in maintaining cellular homeostasis under stress conditions.
Pathways
The ABCF3 protein significantly contributes to the mechanistic target of rapamycin (mTOR) signaling pathway and the integrated stress response pathway. Its interaction with ribosomal proteins suggests a regulatory effect on mTOR signaling which is integral to cell growth and metabolism. ABCF3 also indirectly influences other signaling proteins involved in these pathways such as eukaryotic initiation factors which are key translational regulators.
Studies show ABCF3's involvement in cancer and inflammatory responses. Aberrant expression of ABCF3 links with tumorigenesis influencing cancer cell proliferation and survival. It connects to other cancer-related proteins including mTOR-related proteins highlighting its role in tumor progression. Additionally involvement in inflammatory pathways suggests connections to inflammation-driven conditions where ABCF3 might affect inflammatory regulators.