Abcam, ab258934, Human KNSTRN (SKAP) knockout HEK-293T cell lysate

Size: 1Kit
KNSTRN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 4 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-KNSTRN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
SKAP also known as Src Kinase-Associated Phosphoprotein of 55 kDa is an adaptor protein that plays an important role in T cell activation. It weighs approximately 55 kDa and is prominently expressed in lymphoid tissues where it supports the immune response. Functionally SKAP forms part of signaling cascades that transmit extracellular signals into the cell interior modulating cellular activities.
Biological function summary
SKAP regulates immune cell function by influencing adhesion and migration processes. It often forms part of larger complexes within the immune synapse facilitating interactions between proteins necessary for efficient signaling. SKAP's role in T cells involves partnering with other signaling molecules to strengthen immune responses against infections by promoting cell adhesion.
Pathways
SKAP participates actively in the T-cell receptor (TCR) signaling and integrin-mediated pathways. It associates with proteins such as LFA-1 an important integrin that mediates cell adhesion. SKAP affects the downstream signaling events by modulating key interactions and conformations which are important for effective immune response initiation and regulation.
SKAP has notable connections with autoimmune diseases like rheumatoid arthritis and multiple sclerosis. Its deregulation affects the immune system's balance leading to exacerbated inflammatory responses. Connections to proteins like RapL which interacts with integrins to regulate cell adhesion and migration imply its involvements in pathological conditions involving the immune system's improper regulation.