Abcam, ab258959, Human MAST2 (MAST205) knockout HeLa cell lysate

Size: 1Kit
MAST2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-MAST2, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The target MAST205 also referred to as Microtubule-Associated Serine/Threonine Kinase 205 functions mechanically as a kinase that phosphorylates serine and threonine residues on substrate proteins. With a molecular weight of approximately 205 kDa MAST205 plays an important role in signaling pathways. Researchers have found its expression in diverse tissues including brain and heart where it may influence various cellular processes.
Biological function summary
The kinase activity of this protein controls cell division differentiation and signaling. MAST205 often functions as part of a complex that includes other kinases and regulatory proteins influencing microtubule stability and dynamics. This stability is important for proper cell cytoskeleton structure which is essential for accurate cellular functions and movement.
Pathways
The MAST205 kinase joins in cellular mechanisms such as the Wnt and MAPK signaling pathways. These pathways are central to cellular growth development and response to external signals. MAST205 interacts with proteins like GSK3β and MEK1 that help regulate these pathways therefore influencing downstream signaling and physiological responses.
MAST205 connections have been found to cancer and neurodegenerative disorders. In cancer its dysregulation may alter pathways involving proteins such as β-catenin and lead to unchecked cell proliferation. In neurodegenerative disorders the changes in microtubule dynamics and interactions with proteins like tau can result in neural dysfunction and degeneration. Researchers continue to study MAST205 to understand its role in these pathological conditions fully.