Abcam, ab258990, Human NDUFA6 knockout HeLa cell lysate

Size: 1Kit
NDUFA6 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NDUFA6, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The NDUFA6 protein also known as NADH:Ubiquinone Oxidoreductase Subunit A6 is an essential component of the mitochondrial respiratory chain complex I. Weighing approximately 15 kDa this protein forms a part of the inner mitochondrial membrane mainly expressed in tissues with high energy demands such as the heart brain and skeletal muscle. The protein aids in the initial steps of electron transfer from NADH to ubiquinone which is an important mechanical function in cellular respiration.
Biological function summary
NDUFA6 participates in the assembly and stability of complex I which is the largest of the five complexes in the mitochondrial electron transport chain. By contributing to complex I NDUFA6 plays a pivotal role in ATP production through oxidative phosphorylation. It ensures the effective transfer of electrons and maintains the proton gradient necessary for ATP synthesis. The integration of NDUFA6 within complex I illustrates its biological necessity for proper cellular energy metabolism.
Pathways
NDUFA6 significantly impacts the oxidative phosphorylation pathway and mitochondrial complex I assembly pathway. Its interaction within these pathways influences the overall energy metabolism and cellular respiration efficiency. The protein engages with several partners including NDUFS2 and NDUFV1 which are also integral subunits of complex I. These interactions highlight how NDUFA6 tightly coordinates with other proteins to ensure optimal energy production.
Defects in NDUFA6 have been associated with mitochondrial diseases such as Leber Hereditary Optic Neuropathy and mitochondrial complex I deficiency. These conditions lead to impaired energy metabolism affecting tissues with high energy demands. In these diseases interactions with proteins like NDUFS1 and other complex I subunits become altered disrupting normal mitochondrial function. Understanding these connections emphasizes the importance of NDUFA6 in maintaining mitochondrial health and cellular energy balance.