Abcam, ab259000, Human NPTN knockout HEK-293T cell lysate

Size: 1Kit
NPTN KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon3 and 8 bp deletion in exon3.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 5 bp deletion in exon3 and 8 bp deletion in exon3.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-NPTN, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
Neuroplastin (NPTN) also known as Basigin-like protein 1 plays an important role in various cellular functions. This protein has a mass of approximately 34 kDa as determined by its amino acid sequence. NPTN locates itself on the cell membrane and exhibits expression in multiple tissues especially in the nervous system and skeletal muscles. This protein is known for participating actively in cell adhesion processes which influence neural development and synaptic plasticity.
Biological function summary
Neuroplastin contributes to important cellular processes like calcium homeostasis and synapse formation. As part of the oligomeric receptor complex it facilitates calcium-mediated signaling by forming functional partnerships with other membrane proteins such as plasma membrane Ca2+ ATPases. Neuroplastin's interactions influence dendritic spine morphology and synaptic strength which are essential for proper neuronal communication and memory formation.
Pathways
Neuroplastin functions significantly in the Calcium signaling pathway which regulates numerous cellular activities including muscle contraction and neurotransmitter release. It associates with proteins like PMCA playing a role in calcium ion efflux and maintaining intracellular calcium concentration. Additionally Neuroplastin is involved in the MAP kinase signaling pathway which contributes to cell viability and adaptation to stress.
Neuroplastin shows linkage to conditions such as schizophrenia and hearing loss. Abnormal expression or mutations of NPTN can disrupt neural connectivity and synaptic functions potentially leading to psychiatric disorders. Furthermore studies associate NPTN with Basigin in disorders affecting auditory capacities suggesting possible therapeutic targets for intervention in inner ear function deficits.