Abcam, ab259024, Human PACSIN3 knockout HEK-293T cell lysate

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PACSIN3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4.
Key facts
Cell type:HEK-293T,
Species or organism:Human,
Tissue:Kidney,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 4.

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PACSIN3, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Zygosity-Homozygous, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
PACSIN3 also known as Protein Kinase C and Casein Kinase Substrate in Neurons Protein 3 plays a role in cellular endocytosis and cytoskeleton organization. This protein is about 47 kDa in size. You find PACSIN3 widely expressed in muscle and heart tissues. It contains an F-BAR domain allowing it to bind membranes and promote membrane curvature. This F-BAR domain is a feature of proteins involved in membrane dynamics.
Biological function summary
The role of PACSIN3 extends into various cellular processes related to membrane trafficking where it acts to modulate vesicular transport and actin reorganization. PACSIN3 interacts with other proteins to form complexes that facilitate the internalization and recycling of receptors on the cell surface. Additionally its interaction with other proteins helps regulate cytoskeletal rearrangements essential for various cellular functions.
Pathways
Functions involving PACSIN3 integrate into key signaling pathways such as the endocytic pathway and the actin polymerization pathway. Within these pathways PACSIN3 interacts with proteins like dynamin a GTPase involved in the scission of budding vesicles from the membrane. Additionally it shares functional connections with proteins such as CTTN (cortactin) another actin-binding protein that influences actin polymerization and cellular movement processes.
Several links exist between PACSIN3 and diseases associated with dysregulated endocytosis and impaired cell signaling including muscular dystrophy and heart failure. PACSIN3's relationship with dynamin which is implicated in these conditions illustrates its potential role in the progression or mitigation of these disorders. Connections between PACSIN3 and other signaling proteins such as Rho GTPases further highlight its possible involvement in these pathological states given their importance in actin cytoskeleton dynamics and membrane trafficking.