Abcam, ab259056, Human PPM1H knockout HeLa cell lysate

Size: 1Kit
PPM1H KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.
Key facts
Cell type:HeLa,
Species or organism:Human,
Tissue:Cervix,
Knockout validation:Sanger Sequencing,
Mutation description:Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.,
Disease:Adenocarcinoma

Product details:
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation:
Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10).
This means that the protein of interest is denatured.
If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions:
Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our
limited use license
patent pages

Properties and Storage Information:
Gene name-PPM1H, Gene editing type-Knockout, Gene editing method-CRISPR technology, Knockout validation-Sanger Sequencing, Shipped at conditions-Ambient - Can Ship with Ice, Appropriate short-term storage conditions--20°C, Appropriate long-term storage conditions--20°C

Supplementary Information:
This supplementary information is collated from multiple sources and compiled automatically.
The protein phosphatase Mg2+/Mn2+ dependent 1H (PPM1H) is an enzyme that regulates cellular functions by removing phosphate groups from specific proteins. Known also as protein phosphatase 1H it has a molecular mass of approximately 55 kDa. It is expressed mainly in the brain but also in other tissues such as heart and kidney. PPM1H plays a role in intracellular signal transduction by dephosphorylating target proteins involved in cellular processes.
Biological function summary
PPM1H modulates cellular signaling pathways impacting various physiological processes. It acts independently and does not form part of any known larger protein complex. Within cells PPM1H may interact with substrates involved in important cellular functions such as growth differentiation and apoptosis. Researchers propose that it acts as a regulatory phosphatase influencing cell cycle and stress response at a cellular level.
Pathways
PPM1H is involved in signaling pathways like the TGF-beta and MAPK pathways. In these pathways it interacts with proteins such as SMAD2 and MAPK modulating their activity through dephosphorylation. The TGF-beta pathway plays an important role in regulating cell growth and differentiation while the MAPK pathway is essential for transmitting extracellular signals to cellular responses including gene expression.
PPM1H has been implicated in nervous system disorders like Parkinson's disease and Alzheimer's disease. Studies suggest that altered PPM1H function can disrupt signaling pathways leading to neuronal degeneration. In Parkinson's disease PPM1H's connection to LRRK2 a protein kinase mutated in some hereditary cases shows potential implications in disease progression due to regulatory interactions.